Q1. _______ was found to be the most sensitive in the detection of Salmonella in seafood.

1. Culture Method
2. ELISA
3. PCR assay
4. Immunology based method

ANSWER – C. PCR assay

• Explanation: When compared to culture and ELISA methods for detecting Salmonella in seafood, the PCR assay was shown to be the most sensitive. The enhanced sensitivity of the PCR approach could explain the lower detection rate with culture and ELISA procedures. False-negative PCR findings can also occur when analyzing food samples, which can be caused by interference with target-cell lysis, which is required for nucleic acid extraction, nucleic acid degradation, and/or direct PCR inhibition. As a result, proper controls must be included when using PCR to detect pathogens in food samples.

READ MORE – Food Science & Technology Quiz (15-04-2022)

Q2. _______ remain the most reliable and accurate techniques for food-borne pathogen detection

1. Conventional culture methods
2. Immunology based methods
3. PCB
4. All of the above

ANSWER: A. Conventional culture methods

• Explanation: Traditional culture methods are still the most reliable and accurate ways of detecting foodborne pathogens. Although culture-based methods for detecting single bacteria are found to be typical microbiological procedures, amplification of the signal is necessary by growing a single cell into a colony. L. monocytogenes, S. aureus, Salmonella, Coliforms, E. coli, Campylobacter jejuni, and Yersinia enterocolitica have all been detected using culture methods. Because they rely on the capacity of bacteria to proliferate to visible colonies, certain common methods for detecting L. monocytogenes can take up to 7 days to produce results.

READ MORE – Food Science & Technology Quiz (14-05-2022)

Q3. _______ is perhaps the only technology that has been successfully employed for the detection of bacterial cells, spores, viruses, and toxins alike.

1. PCR
2. Culture Method
3. Colony counting method
4. Immunology based method

ANSWER: D. Immunology based method

• Explanation – Antibody-based immunological detection is perhaps the only approach that has proved successful in detecting bacterial cells, spores, viruses, and poisons. Antigen-antibody binding methods are commonly employed to identify food-borne infections. For the detection of Escherichia coli, Salmonella L,monocytogenes, Staphylococcal enterotoxins, and Campylobacter spp., immunology-based approaches were applied. Because physiological activities in cells are frequently altered in response to stress, the influence of stress on antibody reactions should be thoroughly researched and understood first to assure the reliable detection of foodborne pathogens utilizing antibody-based approaches.

READ MORE – Food Science & Technology Quiz (08-05-2022)

Q4. The main classes of bioreceptors that are widely used in biosensor applications include?

1. Enzymes
2. Antibodies
3. Nucleic acids
4. All of the above

ANSWER: D. All of the above

• Explanation:  Biosensor technologies rely on bioreceptors, or biological recognition elements, to achieve specificity. A bioreceptor is a molecular species that recognizes things using a biological mechanism. They are in charge of attaching the analyte of interest to the sensor so that it can be measured. Bioreceptors can be divided into five major types. Antibodies/antigens, enzymes, nucleic acids/DNA, biological structures/cells, biomimetics, and bacteriophage are among these categories (phage). Bioreceptors include enzymes, antibodies, and nucleic acids, which are commonly utilized in biosensor applications.

READ MORE – Food Science & Technology Quiz (16-04-2022)

Q5. _______ is an analytical device, which converts a biological response into an electrical signal.

1. Bioreceptor
2. Biosensor
3. Transducer
4. None of the above

ANSWER: B. Biosensor

• Explanation – A biosensor is an analytical instrument that converts an electrical signal from a biological response. A bioreceptor or biorecognition element recognizes the target analyte, and a transducer converts the recognition event into a measured electrical signal. A bioreceptor can be a tissue, microbe, organelle, cell, enzyme, antibody, nucleic acid, or biomimetic, for example, and the transduction can be optical, electrochemical, thermometric, piezoelectric, magnetic, or micromechanical, or a combination of the aforementioned.

READ MORE – Food Science & Technology Quiz (14-05-2022)

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