Q1. _______ is a viscous yellowish oil.

1. Purified vitamin A
2. Vitamin B
3. Vitamin C
4. Vitamin K

ANSWER: A. Purified vitamin A

• Explanation: Vitamin A is a thick yellowish oil that has been purified. Most organic solvents, including methanol, ether, acetone, chloroform, and petroleum ether, as well as fatty oils like olive, sesame, and groundnut, are easily soluble in them. Vitamin A, on the other hand, is water-insoluble. It is very vulnerable to oxidizing chemicals and metal ions, as well as ambient oxygen and oxygen carriers. Purified vitamin A is a viscous yellowish oil.

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Q2. Vitamin A assay is carried out by a chemical method called?

1. Antioxidant assay
2. Phenol sulphuric assay
3. Carr Price Reaction
4. None of the above

ANSWER: C. Carr Price Reaction

• Explanation – The test for vitamin A is done using a chemical technique. Carr Price Reaction is the name of this approach. When a saturated solution of antimony trichloride in anhydrous and alcohol-free chloroform is added to vitamin A and carotenoids, the result is a strong blue color. Carr Price Reaction is a reaction that is used to detect Vitamin A. Around 620 nm is where the absorption peaks.

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Q3. ______ is a white, crystalline powder, slightly hygroscopic, of an odor resembling yeast and of bitter taste.

1. Riboflavin
2. Pyridoxine hydrochloride
3. Thiamine hydrochloride
4. Retinol

ANSWER – C. Thiamine hydrochloride

• Explanation: Thiamine hydrochloride is a white, crystalline powder that is slightly hygroscopic, has a yeast-like odora , and has a bitter taste. It dissolves easily in water. Thiamine hydrochloride is soluble in glycerin, methanol, and ethanol (95 percent), but in pure alcohol, acetone, ether, chloroform, benzene, lipids, and fatty oils it is highly insoluble.

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Q4. _______ refers to a group of Cobalt-containing corrinoids known as cobalamins.

1. Vitamin B1
2. Vitamin B2
3. Vitamin B12
4. Biotin

ANSWER: C. Vitamin B12

• Explanation: Cobalamins are a category of cobalt-containing corrinoids that make up vitamin B12. It’s also known as the anti-anemia factor or extrinsic factor. The principal metabolically active molecules in humans and animals are derivatives of hydroxycobalamin, adenosylcobalamin, and methylcobalamin. The active co-enzymic forms are the latter two. Vitamin B12 is synthesized as cyanocobalamin.

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Q5. The chemical methods of the assay are based on color reactions of pyridine for the determination of?

1. Niacin
2. Folic acid
3. Biotin
4. Cyanocobalamin

ANSWER: A. Niacin

• Explanation – The chemical techniques of testing for niacin are based on pyridine color reactions. Nicotinic acid and nicotinamide are transformed to a glutacone aldehyde derivative by cyanogen bromide, which is then condensed with aromatic amines to produce polymethine dyes (yellow coloring reactions). Lactobacillus arabinosus produces lactic acid, which is measured titrimetrically in the microbiological technique.

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